The "3rd Edition of the International Conference on Probiotics and Prebiotics" (Probiotics 2025) is a distinguished event dedicated to advancing research in probiotics, prebiotics, and the microbiome. Focused on the theme "Microbiome Matters: Shaping the Future of Health with Probiotics and Prebiotics," the conference aims to foster collaboration among global experts and drive innovations in gut health and overall well-being. Scheduled for March 27-29, 2025, Probiotics 2025 will take place in Singapore, offering both in-person and virtual participation options to accommodate diverse attendees.